FIG. 3.

Analysis of UQ synthesis in E. coli cells overexpressing carotenoid desaturases CrtN, CrtI, and CrtI14. HPLC profiles of extracts from E. coli cells transformed with the control plasmid pUCMod and the carotenoid desaturases CrtN, CrtI, or CrtI14 on plasmid pUC-CrtN, pUC-CrtI, or pUC-CrtI14. Signals were recorded at 275 nm (A) and 330 nm (B) simultaneously for each of the four extracts to monitor all UQ compounds at 275 nm and show peaks corresponding to desaturated UQ compounds at 330 nm. Peaks are labeled according to the compound numbers shown in Fig. 2. Insets show recorded UV/Vis spectra for individual compound peaks.