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Metabolic tracing of CrtN activity with [14C]IPP. Lyophilized E. coli cells overexpressing IspB (OPP synthase) alone (A) and together with carotenoid desaturase CrtN (B) were radiolabeled with [14C]IPP and nonlabeled FPP. Labeled cells were harvested after 30 min of incubation, extracted, and analyzed by radio-HPLC. A new peak with a shorter retention time than OPP appears in labeled cells expressing CrtN (B), suggesting synthesis of desaturated OPP by CrtN.
