TABLE 2.
Theat (°C) | theat (min) | W/Wtot | E(N) | τr (h) | β | θ (h) | Decimal reduction |
---|---|---|---|---|---|---|---|
50 | 1 | 97/200 | 1.37 | 0.52 | 2.61 | 0.68 | 0 |
5 | 154/200 | 1.91 | −1.27 | 2.51 | 2.47 | 0 | |
20 | 164/199 | 2.11 | 5.29 | 2.30 | 1.67 | 0 | |
30 | 131/199 | 1.63 | 3.93 | 1.95 | 3.15 | 0 | |
40 | 148/199 | 1.83 | 5.52 | 2.02 | 4.39 | 0 | |
52 | 1 | 95/200 | 1.36 | 1.03 | 3.11 | 0.83 | 0 |
5 | 150/199 | 1.86 | 4.79 | 2.27 | 1.83 | 0 | |
10 | 153/200 | 1.89 | 9.44 | 2.08 | 4.82 | 0 | |
20 | 127/200 | 1.59 | 6.98 | 2.59 | 9.69 | 0 | |
30 | 75/200 | 1.25 | 12.10 | 1.70 | 14.27 | 0 | |
40 | 122/200 | 1.54 | 9.14 | 2.02 | 14.92 | 0 | |
53 | 2 | 158/199 | 1.99 | 2.22 | 3.47 | 1.22 | 0 |
4 | 170/199 | 2.25 | 6.26 | 2.84 | 2.09 | 0 | |
6 | 168/199 | 2.20 | 6.18 | 2.61 | 4.31 | 0 | |
8 | 153/199 | 1.91 | 9.26 | 2.20 | 5.73 | 0 | |
10 | 152/199 | 1.89 | 8.84 | 2.05 | 7.00 | 0 | |
20 | 173/199 | 2.34 | 9.90 | 2.48 | 17.21 | 0 | |
30 | 189/198 | 3.24 | 17.00 | 2.53 | 18.23 | 0.5 | |
40 | 164/199 | 2.11 | 20.90 | 2.35 | 21.85 | 1 | |
54 | 2 | 155/198 | 1.95 | 3.18 | 3.41 | 1.17 | 0 |
4 | 174/196 | 2.46 | 4.15 | 3.54 | 3.67 | 0 | |
6 | 138/198 | 1.71 | 13.00 | 1.94 | 9.26 | 0 | |
8 | 157/198 | 1.99 | 11.30 | 1.96 | 12.57 | 0 | |
10 | 129/196 | 1.63 | 16.30 | 1.96 | 15.71 | 0 | |
20 | 195/199 | 3.99 | 13.30 | 3.81 | 13.47 | 1 | |
30 | 128/195 | 1.63 | 31.00 | 1.77 | 30.29 | 2 | |
40 | 61/199 | 1.19 | 26.40 | 1.54 | 42.23 | 3 | |
55 | 4 | 126/199 | 1.58 | 8.77 | 1.82 | 7.49 | 0 |
8 | 131/199 | 1.63 | 20.70 | 1.83 | 43.54 | 0.5 | |
12 | 137/199 | 1.69 | 29.00 | 2.11 | 21.41 | 1.5 | |
18 | 169/199 | 2.23 | 26.80 | 1.87 | 62.23 | 2 | |
25 | 156/185 | 2.20 | 42.10 | 1.65 | 46.55 | 3 | |
30 | 123/195 | 1.58 | 61.50 | 1.64 | 52.08 | 4 | |
62 | 1 | 161/198 | 2.06 | 28.86 | 1.62 | 48.14 | 2 |
1.32 | 165/196 | 2.19 | 45.32 | 1.80 | 56.83 | 3 | |
1.75 | 181/200 | 2.60 | 41.09 | 2.12 | 42.82 | 4 | |
65 | 0.25 | 183/200 | 2.69 | 31.86 | 2.09 | 48.36 | 2 |
0.33 | 176/200 | 2.41 | 41.25 | 1.87 | 55.74 | 3 |
W is the number of wells showing growth, Wtot is the total number of wells taken into account in each experiment, E(N) is the average number of cells per well estimated from the number of empty wells (from a truncated Poisson distribution), τr is the repair time or shift of the distribution, and β and θ are the shape and scale parameters, respectively. The result of the heat treatment for 5 min at 50°C was not taken into account in the secondary modeling because of the negative shift.