FIG. 2.
In vitro and in vivo bioluminescence of selected A. fumigatus strains producing firefly luciferase. (A) Digital picture showing light emission from crude extracts of the transformants B2, B4, B8, C8, C5, and C3 after growth on glucose. The parental wild-type strain CBS144.89 and recombinant luciferase (in the range of 5 to 20 μg) with and without (w/o) the addition of wild-type crude extract served as controls. The addition of crude extract slightly quenched the light emission from the luciferase control. (B) Determination of light emission by use of the IVIS 100 system. Different conidium concentrations (1 × 104, 1 × 105, and 1 × 106) of the wild-type strain (WT) and the three transformants C8, C3, and B4 were inoculated in RPMI medium and incubated for 8 h at 37°C. Light emission was induced by the addition of d-luciferin to the medium, and photons were collected for 1 min. (C) Quantification of light emission of wells shown in panel B by use of Living Image software, version 3.0.