TABLE 1.

Oligonucleotides used in this study

Name	Target gene	Sequence (5′ → 3′)a	Restriction site	Function
PgpdAAfNot_up	gpdA	GCG GCC GCA GAT TCT AGA AGT CCT G	NotI	Amplification of the gpdA promoter
PgpdAAfBglATG	gpdA	AGA TCTCAT TGT GTA GAT TCG TCT GGT AC	BglII	Amplification of the gpdA promoter
Anchored oligo(dT)20	mRNA	NVT TTT TTT TTT TTT TTT TTT T		Generation of cDNA
BglAfGPDcDNA_up	gpdA	AGA TCT GCC ACT CCC AAG GTT GG	BglII	Amplification of gpdA cDNA
HindAfGPDcDNA_do	gpdA	AAG CTT ACT GGG AAT CGA CCT TGG	HindIII	Amplification of gpdA cDNA
LucBgl_up	luc	AGA TCT GAC GCC AAA AAC ATA AAG AAA GG	BglII	Amplification of luc
LucHind_down	luc	AAG CTT ACA CGG CGA TCT TTC CGC	HindIII	Amplification of luc
LucMi_up	luc	GAT GTC CAC CTC GAT ATG TG		Control primer
Not_ptrA_up_for	ptrA	GCG GCC GCT TGA TTA CGG GAT CCC ATT GG	NotI	Amplification of ptrA
Not_ptrA_dow_rev	ptrA	GCG GCC GCG TAT TAT ACT GTC TTT CTT GTT ACA	NotI	Amplification of ptrA
cDNAgpdAAf_up	gpdA	CGT CGA TGC TCC CTC CAA C		RT-PCR with gpdA
cDNAgpdAAf_do	gpdA	GGA ATC GAC CTT GGC GAT G		RT-PCR with gpdA
lucProbe_up	luc	GAT GGA ACC GCT GGA GAG C		luc probe/RT-PCR with luc
lucProbe_mi_rev	luc	CCA AAA CCG TGA TGG AAT GGA AC		luc probe/RT-PCR with luc
AfCitAcode_up	citA	GCA AGG TCA TCG GCG AGG		RT-PCR with citA
AfCitAcode_do	citA	GTT GTC ACC GTA GCC GAG C		RT-PCR with citA
act_Afum_for_II	act1	GCT TTG CTA CGT CGC TCT CG		RT-PCR with act1
act_Afum_rev_II	act1	GGA GGA GCA ATG ATC TTG ACC		RT-PCR with act1

^aBoldface type indicates restriction sites, and italic type indicates the ATG codon in reverse complement orientation.