TABLE 3.

Effects of antibiotics on growth and light emission from the luciferase-producing A. fumigatus strain C3

Growth condition and concn (μg/ml)	Avg value of fungal outgrowtha	RRUb	Relative activityc (%)
Cycloheximide
0	4.94	1.05 × 106 ± 5.22 × 104	100 ± 5.0
2.5	4.00	5.84 × 105 ± 5.80 × 104	56 ± 5.1
5.0	3.02	4.02 × 105 ± 2.01 × 104	38 ± 2.2
10	2.57	1.88 × 105 ± 1.02 × 104	18 ± 0.9
25	1.48	8.31 × 103 ± 4.54 × 102	0.8 ± 0.03
50	1.04	8.21 × 102 ± 1.97 × 102	0.08 ± 0.02
Nystatin
0	4.84	3.02 × 105 ± 7.94 × 103	100 ± 2.6
0.5	4.78	2.54 × 105 ± 1.34 × 104	84 ± 4.8
1.0	4.34	1.91 × 105 ± 2.81 × 103	63 ± 1.2
1.5	2.32	9.54 × 104 ± 4.32 × 103	32 ± 1.0
2.0	1.90	6.35 × 104 ± 5.89 × 102	21 ± 0.2
3.0	1.08	4.10 × 104 ± 3.17 × 103	14 ± 0.6

^aMicroscopic growth analysis after incubation in the presence of antibiotics. Classification was performed by removing an aliquot and evaluating at least 100 conidia grown under each condition at a ×400 magnification. Values are classified as follows: 5, branching hyphae; 4, elongated hyphae without branching; 3, germ tubes; 2, swollen conidia; 1, resting conidia. All experiments were performed in triplicate.

^bFor experiments with cycloheximide, relative response units (RRU) are given as the sums of three cycles of 5 s each, calculated after 5 min of preincubation. For experiments with nystatin, relative response units are given as the averages from 2-s intervals, determined between 4 and 10 s directly after d-luciferin addition. All experiments were performed in triplicate, and standard deviations are given.

^cAll experiments were performed in triplicate, and standard deviations are given.