TABLE 2.
Effect of MAb concentration on staining of A. anophagefferensa
| FITC-MAb concn (MAb:sample) | Mean green fluorescence (arbitrary units)b |
|---|---|
| 1:100 | 698* |
| 1:500 | 382 |
| 1:1,000 | 302* |
| 1:5,000 | 66 |
| 1:10,000 | 43* |
| 1:20,000 | 29 |
a
A. anophagefferens cells were diluted 1:10 in 10 mM PBS-T and tested over a range of FITC-MAb concentrations. All incubations were performed for 30 min in the dark at room temperature. Values represent the means of the green fluorescence (FL1) signal for the A. anophagefferens gated region.
b
Asterisks indicate concentrations at which units of mean green fluorescence of stained cells differed from that of more dilute concentrations based on pair-wise bootstrap-t analysis of means (P < 0.05).