TABLE 3.

Diol dehydratase activities of cell-free protein extracts of strains grown in media with and without 1,2-PD^a

Strain	Diol dehydratase activity (avg units/mg total cell protein ± SD)c		P valueb
	+1,2-PD	−1,2-PD
Lin11	6.7 × 10−2 ± 2.1 × 10−2	4.0 × 10−3 ± 0	<0.01
Lin11Δ1118	3.0 × 10−3 ± 0	4.0 × 10−3 ± 1.0 × 10−3	>0.05
Lin11Δ1118/pJX1118	1.3 × 10−2 ± 3.0 × 10−3	7.0 × 10−3 ± 3.0 × 10−3	<0.05

^aValues are expressed as average units of diol dehydratase activity per mg of total cellular protein ± standard deviations (SD) in medium with (+) and without (−) 1,2-PD (n = 3; n = 4 for Lin11 in medium +1,2-PD). One unit of diol dehydratase activity is equivalent to the amount of enzyme that converts 1 μmol/min of 1,2-PD to propionaldehyde.

^bP values were calculated to determine if enzyme activities differed significantly due to the addition of 1,2-PD to the medium.

^cP values were calculated to determine if enzyme activities differed significantly between strains; with 1,2-PD, the P value in a comparison of strains Lin11, Lin11Δ1118, and Lin11Δ1118/pJX1118 was <0.01, and without 1,2-PD, the P value among these strains was >0.05.