TABLE 4.
Primers used for real-time RT-PCR analysis of pdu gene transcript levelsa
| Primer | Sequence |
|---|---|
| pocR (lin1114) | |
| F | AAATGTCGCTCGCAAAAGAT |
| R | TAGCTCGTTTGGGTGAATCC |
| pduA (lin1115) | |
| F | GAGGGGATGTAGGTGCAGTT |
| R | ATGCGGACGAGGAATAACAT |
| pduB (lin1116) | |
| F | GGCCTAGTGATTGCGAATGT |
| R | CACCAGTACGGGCTCCTAAA |
| pduC (lin1117) | |
| F | ACAATATGTTTGCGGGTTCC |
| R | ACTGGCGTTAAACCACCATC |
| pduD (lin1118) | |
| F | CTTACCGGGCAATTGGTAAA |
| R | AATTTTGGACGAGCCATTTG |
| pduE (lin1119) | |
| F | AAAATGCAAGCGCAAGTAGC |
| R | TACGCTCATCTGGAACAACG |
| pduG (lin1120) | |
| F | AGCGCGTTTTTGTAACGAAT |
| R | GGCTGATCCTCCGACAATAA |
| pduH (lin1121) | |
| F | TTTGGTTGGAATTACGCTCA |
| R | TGCGCAAAAACGATATTCTG |
| pduK (lin1122) | |
| F | ACAAACGGCAATAGCCTCAG |
| R | GGCACGATTTCTTCTTTTGG |
| pduJ (lin1123) | |
| F | TGATGTTGGCGCAGTAAAAG |
| R | CATTGTGTGGACGTGGGATA |
| pduL (lin1124) | |
| F | TAAAGCAACCTGGCGAATTT |
| R | AAAATTTTCGAAGCGGACCT |
| eutJ (lin1125) | |
| F | TACCGCGAGTCCGTAGAAGT |
| R | CCAGGTGGAATAGCTCCAGA |
| pduM (lin1126) | |
| F | ATTCGTGCCGCAAATAAAAC |
| R | CGAACTGTCCCACTTTCCAT |
| pduN (lin1127) | |
| F | GAACATGTGCGCTCAGAAGA |
| R | CTGCAAATACGCTCCTAGCC |
| pduO (lin1128) | |
| F | TCAGGTGCAGGTTGTCTCAG |
| R | AGCGTGTTCCATTCGGTAAT |
| pduP (lin1129) | |
| F | CGACAAGAGCAGTTGCAGAG |
| R | ATCTTCTACCCCTGGCGTTT |
| pduQ (lin1130) | |
| F | CAAATGCCACGAACATTGAC |
| R | CAAGCAAGCGTCTTTCAGTG |
| pduF (lin1131) | |
| F | TTGGCGGAATTTATTGGAAC |
| R | CCCAGTTTGCTCCTTGTGAT |
| pduW (lin1132) | |
| F | CGGATGTTTACTGGCCAAAT |
| R | CACCAACTCCAGCCGTAAAT |
| cobD (lin1133) | |
| F | CGTATTGCCTCGTTTCATCA |
| R | TTTGCGCCTTGGTTACTTTT |
| pdu X (lin1134) | |
| F | AAAAGGGTCCGTGATTGAAA |
| R | TTCTTGCCGATATGTTGCAG |
| 16S rRNA | |
| F | AAGCAACGCGAAGAACCTTA |
| R | TGCACCACCTGTCACTTTGT |
a
F, forward primer; R, reverse primer. Primers were designed using the L. innocua CLIP11262 genome sequence available at the ListiList server (http://genolist.pasteur.fr/ListiList).