TABLE 5.
Relative levels of pdu mRNAs in L. innocua Lin11a
| Gene | Relative fold induction of mRNA (range [lowest-highest])
|
|
|---|---|---|
| Lin11 in LB medium − 1,2-PD | Lin11 in LB medium + 1,2-PDb | |
| pocR (lin1114) | 1.0 (0.6-1.6) | 3.1 (2.1-4.5)* |
| pduA (lin1115) | 1.0 (0.8-1.2) | 84 (67-110)* |
| pduB (lin1116) | 1.0 (0.7-1.4) | 75 (51-100)* |
| pduC (lin1117) | 1.0 (0.8-1.2) | 43 (35-54)* |
| pduD (lin1118) | 1.0 (0.6-1.8) | 12 (9.2-17)* |
| pduE (lin1119) | 1.0 (0.9-1.1) | 13 (11-15)* |
| pduG (lin1120) | 1.0 (0.8-1.3) | 15 (13-19)* |
| pduH (lin1121) | 1.0 (0.7-1.3) | 12 (9.4-16)* |
| pduK (lin1122) | 1.0 (0.8-1.2) | 11 (8.8-13)* |
| pduJ (lin1123) | 1.0 (0.9-1.1) | 7.6 (6.6-8.9)* |
| pduL (lin1124) | 1.0 (0.8-1.2) | 7.6 (6.0-9.8)* |
| eutJ (lin1125) | 1.0 (0.8-1.3) | 7.8 (6.4-9.6)* |
| pduM (lin1126) | 1.0 (0.8-1.2) | 5.2 (4.5-6.0)* |
| pduN (lin1127) | 1.0 (0.8-1.2) | 5.5 (4.7-6.6)* |
| pduO (lin1128) | 1.0 (0.9-1.2) | 3.2 (2.8-3.8)* |
| pduP (lin1129) | 1.0 (0.5-1.8) | 5.0 (3.7-6.7)* |
| pduQ (lin1130) | 1.0 (0.9-1.2) | 4.2 (3.5-5.1)* |
| pduF (lin1131) | 1.0 (0.6-1.7) | 3.2 (1.4-7.0)* |
| pduW (lin1132) | 1.0 (0.8-1.3) | 1.1 (0.7-1.9) |
| cobD (lin1133) | 1.0 (0.6-1.5) | 0.9 (0.7-1.1) |
| pduX (lin1134) | 1.0 (0.7-1.5) | 0.9 (0.7-1.2) |
a
The values shown are averages of relative levels of pdu mRNAs in L. innocua Lin11 grown aerobically in LB/vitamin B12 medium with (+) or without (−) 52 mM 1,2-PD, obtained using three independent RNA preparations. Transcript levels were measured in triplicate for each RNA preparation. The cycle threshold values for PCR products were compared to those measured in strain lin11 grown in LB medium without 1,2-PD to determine differences. Values in parentheses signify the lowest and highest values, respectively, measured for each mRNA.
b
Asterisks indicate values that are significantly different between the two media (P < 0.01).