TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or phenotype | Source (reference) |
|---|---|---|
| Strains | ||
| B. subtilis | ||
| 168 | trpC2 | Laboratory stock |
| WN1038 | Spontaneous Trp+ revertant of 168 | Laboratory stocka |
| WN1063 | yycR::cat; Cmr | pWN1057 → WN1038 tfb |
| WN1075 | ydjL::cat; Cmr | pWN1071 → WN1038 tf |
| E. coli | ||
| DH5α | deoR endA1 gyrA96 hsdR17 (rk− mk+) recA1 relA1 supE44 thi-1 Δ(lacZYA-argFV169) φ80lacZΔM15 F− λ− | H. Jost (9) |
| WN1057 | DH5α (pWN1057); Apr | This study |
| WN1071 | DH5α (pWN1071); Apr | This study |
| Plasmids | ||
| pBGSC-6 | Replicates in E. coli (Apr); integration vector in B. subtilis (Cmr) | BGSCb (5) |
| pWN1057 | pBGSC-6 with internal yycR fragment | This study |
| pWN1071 | pBGSC-6 with internal ydjL fragment | This study |
a
Strain WN1038 was donated to the BGSC as strain 1A874.
b
Abbreviations: BGSC, Bacillus Genetic Stock Center; tf, transformation.