TABLE 3.
Category and gene | Functionb | Fold changec
|
|
---|---|---|---|
Microarrayd | qRT-PCRe | ||
Gene encoding proteins involved in transcription | |||
lmo0153 | Oligopeptide ABC transporter; oligopeptide-binding protein | 4.9 | 163.1 |
lmo0154 | Oligopeptide ABC transporter; permease protein | 4.7 | 14.0 |
lmo1443 | Transporter; NRAMP family | 4.4 | 49.1 |
lmo1875 | ABC transporter; manganese-binding protein | 16.7 | 435.5 |
lmo1876 | Manganese ABC transporter; permease protein | 11.5 | 290.0 |
lmo1877 | Manganese ABC transporter; ATP-binding protein | 12.3 | 71.8 |
Gene encoding proteins involved in transcription | |||
lmo0914 | RNA polymerase δB factor | 2.1 | 3.8 |
Gene encoding proteins involved in amino acid biosynthesis | |||
lmo0234 | Cysteine synthase A | 3.7 | 14.8 |
Gene encoding proteins involved in energy metabolism | |||
lmo1365 | Glycine cleavage system T protein | 3.7 | 10.5 |
Gene encoding proteins involved in central intermediary metabolism | |||
lmo0977 | Glucosamine-6-phosphate isomerase | 3.2 | 3.3 |
Gene encoding proteins involved in protein synthesis | |||
lmo2484 | Ribosomal subunit interface protein | 2.4 | 5.4 |
Gene encoding proteins involved in toxin production and resistance | |||
lmo0963 | Peroxide resistance protein Dpr | 2.6 | 8.0 |
Gene encoding proteins involved in cell division | |||
lmo2064 | Cell division protein FtsZ | 2.7 | 5.7 |
Genes encoding hypothetical or unknown proteins | |||
lmo0243 | ATP:guanido phosphotransferase family protein | 2.3 | 2.4 |
lmo0703 | Hypothetical protein | 4.0 | 2.7 |
lmo0899 | LysM domain protein | 4.2 | 57.2 |
lmo0974 | Conserved hypothetical protein | 3.1 | 50.4 |
lmo0975 | Conserved hypothetical protein | 2.9 | 34.2 |
lmo1520 | Conserved hypothetical protein | 2.5 | 4.6 |
lmo1622 | Conserved hypothetical protein | 2.2 | 6.9 |
lmo1996 | Conserved hypothetical protein | 3.4 | 22.7 |
lmo2063 | Conserved hypothetical protein | 2.6 | 3.3 |
lmo2190 | Conserved hypothetical protein | 2.5 | 7.7 |
lmo2459 | PspC domain protein | 4.0 | 31.6 |
lmo2460 | Conserved hypothetical protein | 4.3 | 26.7 |
lmo2541 | Conserved hypothetical protein | 3.2 | 31.0 |
Only the genes that met the stringent criteria for being upregulated in milk (i.e., change of more than twofold; P < 0.01) are listed here.
Gene functions are based on annotations provided by TIGR (http://cmr.tigr.org/tigr-scripts/CMR/CmrHomePage.cgi).
Change indicates the transcript ratios between L. monocytogenes F2365 grown in milk and BHI medium at 4°C as determined by microarray and real-time PCR.
Numbers are average values from four independent experiments.
Numbers are average values from three independent experiments.