TABLE 4.
Category and gene | Functionb | Fold changec
|
|
---|---|---|---|
Microarrayd | qRT-PCRe | ||
Genes encoding proteins involved in protein synthesis | |||
lmo1352 | Ribosomal protein L3 | −5.0 | −3.3 |
lmo1808 | Ribosomal protein L20 | −2.4 | −3.4 |
lmo1814 | Ribosomal protein L19 | −3.1 | −2.2 |
lmo1824 | Ribosomal protein S16 | −2.8 | −3.2 |
lmo1844 | Ribosomal protein L28 | −6.5 | −3.7 |
lmo2078 | Ribosomal protein L32 | −5.3 | −5.7 |
lmo2521 | Ribosomal protein L31 | −3.5 | −2.4 |
lmo2846 | Ribosomal protein L34 | −3.4 | −2.8 |
Gene encoding proteins involved in cellular processes | |||
lmo1381 | Cold shock domain family protein | −2.4 | −2.2 |
Gene encoding proteins involved in regulatory function | |||
lmo1265 | Putative transcriptional regulator | −4.0 | −4.7 |
Gene encoding proteins involved in cell envelope | |||
lmo2209 | Putative membrane protein | −2.4 | −2.5 |
Gene encoding proteins involved in transport and binding | |||
lmo1264 | Putative transporter | −8.3 | −16.3 |
Gene encoding proteins involved in energy metabolism | |||
lmo1946 | Formate acetyltransferase | −4.8 | −3.5 |
Gene encoding hypothetical or unknown proteins | |||
lmo2182 | Hypothetical protein | −3.5 | −2.1 |
Only the genes that met the stringent criteria for being downregulated in milk (i.e., change of twofold; P < 0.01) are listed here.
Gene functions are based on annotations provided by TIGR (http://cmr.tigr.org/tigr-scripts/CMR/CmrHomePage.cgi).
Change indicates the transcript ratios between L. monocytogenes F2365 grown in milk and BHI medium at 4°C as determined by microarray and real-time PCR; negative values indicate transcript levels that are lower in milk than in BHI broth (e.g., a value of −3.2 indicates a 3.2-fold-lower transcript level in milk than in BHI medium).
Numbers are average values from four independent experiments.
Numbers are average values from three independent experiments.