TABLE 4.

L. monocytogenes strain F2365 genes downregulated in skim milk at 4°C as identified by microarray^a and real-time PCR analysis

Category and gene	Functionb	Fold changec
		Microarrayd	qRT-PCRe
Genes encoding proteins involved in protein synthesis
lmo1352	Ribosomal protein L3	−5.0	−3.3
lmo1808	Ribosomal protein L20	−2.4	−3.4
lmo1814	Ribosomal protein L19	−3.1	−2.2
lmo1824	Ribosomal protein S16	−2.8	−3.2
lmo1844	Ribosomal protein L28	−6.5	−3.7
lmo2078	Ribosomal protein L32	−5.3	−5.7
lmo2521	Ribosomal protein L31	−3.5	−2.4
lmo2846	Ribosomal protein L34	−3.4	−2.8
Gene encoding proteins involved in cellular processes
lmo1381	Cold shock domain family protein	−2.4	−2.2
Gene encoding proteins involved in regulatory function
lmo1265	Putative transcriptional regulator	−4.0	−4.7
Gene encoding proteins involved in cell envelope
lmo2209	Putative membrane protein	−2.4	−2.5
Gene encoding proteins involved in transport and binding
lmo1264	Putative transporter	−8.3	−16.3
Gene encoding proteins involved in energy metabolism
lmo1946	Formate acetyltransferase	−4.8	−3.5
Gene encoding hypothetical or unknown proteins
lmo2182	Hypothetical protein	−3.5	−2.1

^aOnly the genes that met the stringent criteria for being downregulated in milk (i.e., change of twofold; P < 0.01) are listed here.

^bGene functions are based on annotations provided by TIGR (http://cmr.tigr.org/tigr-scripts/CMR/CmrHomePage.cgi).

^cChange indicates the transcript ratios between L. monocytogenes F2365 grown in milk and BHI medium at 4°C as determined by microarray and real-time PCR; negative values indicate transcript levels that are lower in milk than in BHI broth (e.g., a value of −3.2 indicates a 3.2-fold-lower transcript level in milk than in BHI medium).

^dNumbers are average values from four independent experiments.

^eNumbers are average values from three independent experiments.