TABLE 2.
Comparison of predicted RNase III sites of bacteriophages φYeO3-12 and φSG-JL2
| Name of putative RNase III site | Range | ΔG (kcal/mol) | Sequence of predicted stem-loopa |
|---|---|---|---|
| φYeO3-12 R0.3 | 956-1009 | −17.6 | UAAGCGAAUAACUCAAGGUCGCACUGAAAGCGUGGCCUUUAU/GAUAUUCACUUA |
| T3 R0.3 | 822-875 | −17.6 | —————————————————————————————————————/——————————— |
| φSG-JL2 R0.3 | 969-1022 | −17.6 | —————————————————————————————————————/—————————— |
| φYeO3-12 R0.45 | 1490-1545 | −21.5 | GUAAGUGUUAAACUCAAGGUCGCUCCAUGCGAGUGGCCUUUAU/GAUUAUCACUUAU |
| T3 R0.5 | 1359-1408 | −20.8 | ...————————————————AU——A—G————..————————/—————A——.——.. |
| φSG-JL2 R0.45 | 1662-1717 | −26.5 | ——————A——G———————————A—UG—AU—U—————————/————————————— |
| φYeO3-12 R1 | 3245-3293 | −20.7 | GAGUCUUUUCUUACAGGUCAUCAUGUGGUGGCCUGAAU/AGGAACGAUUU |
| T3 R1 | 2908-2956 | −20.5 | —————————————————————————A———————/——————————— |
| φSG-JL2 R1 | 3628-3676 | −20.9 | ——————A—————————————————AUC——————/————U—————— |
| φYeO3-12 R1.1 | 6340-6391 | −20.4 | GAGAGUUAAACUUAAGGUCAUCACCGACGGUGGCCUUUGU/GAUUAACUUUC |
| T3 R1.1 | 6003-6053 | −20.4 | ——————————————————————A————————————/—————————— |
| φSG-JL2 R1.1 | 6972-7022 | −20.2 | ———————GC——C———————————————————————/—————————— |
| φYeO3-12 R1.3 | 6856-6896 | −18.2 | GAAUCCU/UAAGGUCACUU AACAUGAGUGGCCUUUGU/GAUUC |
| T3 R1.3 | 6519-6558 | −16.9 | ——————/——————————U————..——————C——/————— |
| φSG-JL2 R1.3 | 7488-7527 | −16.3 | —————/————————UC——U———UG————————-/——-——— |
| φYeO3-12 R3.8 | 11350-11377 | −14.9 | UAAAGGGAGACUUAACGGUUUCCCUUUG |
| T3 R3.8 | 10616-10642 | −13.7 | ———————————————.————————— |
| φSG-JL2 R3.8 | 11564-11591 | −14.9 | ————————————————————————— |
| φYeO3-12 R4.7 | 13706-13754 | −21.7 | AAGUGAUAAACUCAAGGUCGCCCAAGGGUGGCCUUUAU/GAUUAUCAUUU |
| T3 R4.7 | 12970-13018 | −21.7 | ————————————————————————————————-—/———————--- |
| φSG-JL2 R4.7 | 13920-13968 | −21.7 | —————————————————————————————-———-/———------- |
| φYeO3-12 R6.5 | 17901-17971 | −25.9 | AAGUGAUAAACUCAAGGCUCUCUGUA UUAACCCUCACUAAAGGGAAGAGGGAGCCUUUAU/GAUUAUUACUU |
| T3 R6.5 | 17135-17206 | −26.3 | —————————————————————AC—A——————————————————————————————/———-—————— |
| φSG-JL2 R6.5 | 17812-17882 | −24.8 | ————————C———————————————————————————-————————————————/———-—————— |
| φYeO3-12 R13 | 26211-26248 | −21.0 | GUCUCCCUGUGGUGAAUUAACCCUCACUAAAGGGAGAC |
| T3 R13 | 25443-25480 | −19.9 | ————————C————————————————————————— |
| φSG-JL2 R13 | 25848-25885 | −21.0 | —————————————————————————————————— |
| φYeO3-12 R18.5 | 36435-36488 | −23.4 | UAAGUGACAUACUCAAGGUUCUCCACUCGGGGGAGCCUUUAU/GGAUGUUAUUUG |
| T3 R18.5 | 35035-35085 | −23.6 | AC————U——G———————.————.UA—.——UG——————/———————C——GU |
| φSG-JL2 R18.5 | 35677-35729 | −23.5 | —————————————————————————————.——————/———————————— |
a
Slashes indicate cleavage sites; periods indicate deletions; homologous nucleotides are represented by dashes.