TABLE 3.
Frequencies of restriction enzyme and methylase recognition sites in the genomes of φSG-JL2, φYeO3-12. and T3
| R-M system/family | Enzyme | Recognition sequence | No. of recognition sites (location of first recognition site)
|
|||
|---|---|---|---|---|---|---|
| φSG-JL2 (1050-1505)a | φYeO3-12 (1035-1490) | T3 (901-1359) | T7 (925-1276) | |||
| Methylase | Dcm | CC(A/T)GG | 0 | 0 | 2 (19053) | 2 (2366) |
| Methylase | Dam | GATC | 5 (921) | 3 (7382) | 10 (2380) | 6 (8312) |
| Type I/A | EcoKI | AAC(N)6GTGC | 7 (3383) | 5 (3110) | 4 (5490) | 4 (15161) |
| Type I/A | StySBI | GAG(N)6RTAYG | 1 (23718) | 2 (7562) | 2 (3295) | 7 (1491) |
| Type I/A | StySPI | AAC(N)6GTRC | 8 (3383) | 9 (3000) | 7 (2663) | 9 (6356) |
| Type I/B | EcoAI | GAG(N)7GTCA | 6 (9106) | 5 (8232) | 5 (8162) | 0 |
| Type I/B | StySKI | CGAT(N)7GTTA | 2 (3953) | 2 (3570) | 1 (3233) | 0 |
| Type I/D | StySBLI | CGA(N)6TACC | 3 (10375) | 6 (5091) | 7 (4754) | 13 (2107) |
| Type III | EcoP15I | CAGCAG/CTGCTG | 4 (9724)/40 | 4 (8543)/38 | 5 (1938)/47 | 0/36 |
| Type III | StyLTI | CAGAG/CTCTG | 11 (14056)/78 | 13 (12520)/72 | 14 (4795)/63 | 15 (2287)/72 |
a
Location of gp0.3 (SAMase).