FIG. 1.
SDS-PAGE analysis of purified recombinant proteins and CD spectra. (A) Recombinant proteins MPL17 and MPL21 were expressed in E. coli BL21(DE3) and BL21 SI, respectively, and purified from the supernatant of the bacterial cell lysate by using Ni2+-charged chelating Sepharose columns. Lane 1, MPL17; lane 2, MPL21; lane M, protein molecular mass marker. (B) Far-UV spectrum by CD spectroscopy of 20 μM recombinant proteins obtained in 10 mM sodium phosphate buffer. The y axes indicate ellipticity values (to be multiplied by 103) in degrees cm2 dmol−1.