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. 2008 Sep 17;15(11):1715–1722. doi: 10.1128/CVI.00214-08

FIG. 2.

FIG. 2.

Recognition of recombinant proteins MPL17 and MPL21 by IgG antibodies in sera from individuals diagnosed with leptospirosis. (A) Percentage of positive sera (responders) determined by ELISA with recombinant proteins MPL17 and MPL21 and serum samples from patients with both phases of the disease tested at a dilution of 1:100. Serum was considered MAT positive or MAT negative if agglutination was detected when the sera were tested for their reactivities with isolates of the 22 serovars (see Materials and Methods). The cutoff values were defined as the mean plus 3 standard deviations obtained for sera from six healthy individuals. No statistically significant difference in the frequency of positive sera for recombinant proteins MPL17 and MPL21 in the different groups of individuals was observed. (B) Comparison of the antibody titers of serum samples that recognize recombinant proteins MPL17 (left) and MPL21 (right) and that were obtained by MAT. The symbols represent the titer of each serum sample. The solid lines represent the geometric mean antibody titers in each group. Significance was assessed by comparison with antibody titers to MPL17 and those obtained by MAT by use of the unpaired t test (*, P < 0.05).