FIG. 8.

Kinetics of ethanol production of the K. lactis wild-type strain MW270-7B (▵) and its ΔKlhap1 isogenic mutant (□). Cells were grown at 28°C in 50 ml YP-5% glucose contained in 500-ml flasks (upper panels) or 100-ml flasks (lower panels). Aliquots were taken for analysis of cell OD₆₀₀ (A and D), glucose consumption (B and E), and ethanol production (C and F). Other details are described in Materials and Methods. The values are means of analysis for two flask cultures. Bars indicate the up and down variations.