FIG. 2.

CWP-polysaccharide complexes in the cell wall of C. glabrata. (A and B) SDS-PAGE analysis of cell wall material extracted as indicated from isolated walls of C. glabrata grown in SC medium. Gels were analyzed by silver staining or by blotting and probing with the lectin ConA or anti-ScCwp1 antiserum (A) or with anti-1,6-β-glucan antiserum (B). (C) Identified cross-links between proteins and β-glucans in the cell wall of C. glabrata. The extraction methods used to solubilize CWPs are indicated. (D) Cell wall proteins identified in mild-alkali extracts by LC/MS/MS.