FIG. 3.

Effects of increased intrinsic H₂O₂ on hyphal differentiation. Cells were grown in YPD medium containing 0.2 and 1 mM H₂O₂ for 6 h, washed, and resuspended in Hank's balanced salt solution. After the addition of CM-H2DCFDA (10 μM final), the cells were further incubated at RT for 10 min. (A) Images of DCF fluorescence were taken by using a confocal microscope with excitation and emission wavelengths at 488 nm and 520 nm, respectively. (B) Relative concentrations of intracellular H₂O₂ were derived from the confocal microscope-aided integration of fluorescence signal intensity within a scope. (C) Efficiency of hyphal differentiation at 0.2 mM H₂O₂ was determined as described in the legend to Fig. 1C. CAT1-R represents the strain into which the functional CAT1 gene was introduced.