FIG. 4.

Effects of ascorbic acid on hyphal differentiation in FBS-treated cells. Wt cells were grown in YPD in the absence (−) or presence (+) of 10% FBS for 30 min, followed by supplementation with 50 mM or 100 mM ascorbic acid. A portion of the cells was removed to take light microscopic images (A). For the rest of cells, fluorescence images (B) were taken, and the relative concentrations of intracellular H₂O₂ (C) were determined as described in the legend to Fig. 3.