FIG. 5.

Effects of ascorbic acid on hyphal differentiation. After the wt and tsa1Δ, cat1Δ, and tsa1Δ cat1Δ mutant cells were grown in YPD medium supplemented with 4 concentrations of exogenous H2O2 for 30 min at 30°C, 100 mM ascorbic acid was added, and the cells were further grown for 6 h at 30°C, the cultures were observed with a light microscope (magnification, ×400).