FIG. 3.
Chromatography of Aphanomyces euteiches chitosaccharides on a Bio-Gel P-60 column. The chitosaccharides were prepared by multistep digestion of KOH-MeOH cell walls with (1→ 3)-, (1→ 4)-, and (1→ 6)-β-glucanases. V0, void volume, determined with dextran 2000; Vt, total volume of the column. The positions of peaks of dextran standards of the respective molecular masses are shown by vertical arrows. The GlcNAc and Glc concentrations of the fractions were determined by colorimetry using the anthrone reagent and HPAEC-PAD analysis after acid hydrolysis, respectively.