FIG. 5.

Southern hybridization analysis of Aphanomyces euteiches chitin synthase genes. Genomic DNA (100 μg) digested with either ScaI (lane 1), EcoRI (lane 2), or KpnI (lane 3) was analyzed on a 1% agarose gel, transferred to a nylon membrane, and hybridized with a radiolabeled AeCHS1 probe (Aechs1) or AeCHS2 probe (Aechs2). The arrow shows a KpnI restriction fragment hybridizing with the AeCHS1 that was predicted from sequence analysis to generate a 1,080-bp fragment. The positions of molecular size standards (MS) (in base pairs) are shown to the left of the gels.