FIG. 7.

Phylogeny of oomycete chitin synthases. The A. euteiches CHS amino acid sequences were aligned with other oomycete CHS sequences, such as Phytophthora and Saprolegnia CHS sequences. The phylogenetic tree was constructed with the Mega version 3.1 software using minimum evolution (ME), neighbor-joining (NJ), and maximum parsimony (MP) methods (Poisson correction for multiple amino acid substitutions; 1,000 random bootstrap replicates). All three methods produced similar topologies; this figure shows the ME tree. Numbers on each branch represent bootstrap values of ME/NJ/MP analyses. The Emericella nidulans CHSB sequence was employed as an outgroup taxon.