FIG. 2.

ATP production by oxidative phosphorylation is severely affected in mitochondria of MIX RNAi cells. ATP production was measured in digitonin-extracted mitochondria. The three ATP-producing pathways were triggered by the addition of ADP plus one of the following substrates: succinate, α-ketoglutarate, and pyruvate-succinate. ATP production in mitochondria isolated from noninduced cells and tested without any additions (None) was set to 100%. All other values in each panel are means expressed as percentages of this sample. Malonate (Mal.) was used to inhibit ATP production by oxidative phosphorylation, and atractyloside (Atract.) was used to inhibit the import of ADP into mitochondria.