. 2008 Sep 5;7(11):1994–2003. doi: 10.1128/EC.00204-08

TABLE 2.

Proteins found in tagged MIX and cyt c oxidase complexes^a

Group	T. brucei gene	No. of predicted transmembrane domains^b	Molecular mass (kDa)	No. of unique peptides detected with^c:					Possible function and/or similarity (E value)
Group	T. brucei gene	No. of predicted transmembrane domains^b	Molecular mass (kDa)	TAPMIX	TAPCOXIV	TAPCOXV	TAPTb2320	TAPTb1900	Possible function and/or similarity (E value)
A	Tb927.3.1890	NI	33.2	√	√	√	√	ND	Hypothetical protein; Tryp^j specific
	Tb927.5.3040^e	1	23.1	√	1	ND	1	ND	Hypothetical protein; Tryp specific
	Tb09.211.1750	1	34.3	√	√	√	√	1	mt carrier protein (1e−100)
	Tb09.211.4880	1	42.7	√	√	√	√	ND	Cyclophilin isomerase (6e−09)
	Tb11.01.3860	NI	18.4	√	√	√	√	ND	Hypothetical protein; Tryp specific
	Tb11.02.2460	1	50.4	√	√	√	√	ND	Similarity to Homo Sec63 (0.063)
	Tb11.03.0110	1	41.7	√	1	1	ND	ND	DnaJ chaperon (5e−14)
B	Tb927.1.4100^d^,^f	NI	40.5	√	√	√	√	√	cyt c oxidase subunit IV; Tryp specific
	Tb09.160.1820^d^,^g	NI	22.2	√	√	√	√	√	cyt c oxidase subunit V; Tryp specific
	Tb11.0400^d	1	27.7	√	√	√	√	√	Hypothetical protein; Tryp specific
	Tb11.01.4702^d	NI	13.7	√	√	√	√	√	cyt c oxidase subunit X; Tryp specific
	Tb10.70.2320^d^,^h	NI	26	1	√	√	√	√	Hypothetical protein; Tryp specific
	Tb09.211.1900ⁱ	1	29.6	ND	√	√	√	√	Hypothetical protein; Tryp specific
	Tb09.211.4740	1	44.5	ND	√	√	√	√	Saccharomyces Oms1 (4e−19)
	Tb10.70.1890	NI	51.2	ND	√	√	√	√	Hypothetical protein; Tryp specific
	Tb927.7.6990	1	32.7	ND	√	√	√	√	Hypothetical protein; Tryp specific
	Tb11.01.1900	1	30.3	ND	√	√	√	√	Hypothetical protein; Tryp specific
	Tb11.46.0006	2	50.3	ND	√	√	√	√	Hypothetical protein; Tryp specific
C	Tb927.3.1940	1	49.4	ND	√	√	√	√	Hypothetical protein; conserved (3e−06)
	Tb927.5.2580	NI	44.3	ND	√	√	√	√	Hypothetical protein; Tryp specific
	Tb09.v1.0420	1	23	1	√	√	√	√	Hypothetical protein; Tryp specific
	Tb09.160.1140	1	37.5	1	√	√	√	√	SCO1/SCO2 (5e−45)
	Tb10.05.0050	1	18.5	ND	√	√	√	√	Hypothetical protein; Tryp specific
	Tb10.70.5120	NI	39.0	ND	√	√	√	√	Putative malate dehydrogenase (8e−42)

Only proteins identified in more than three experiments and by at least two unique peptides are shown. Proteins likely to be contaminants (including several ribosomal proteins, translation factors, heat shock proteins, alpha- and beta-tubulin, and a number of mt proteins) are listed in Table S2 in the supplemental material.

Number of predicted transmembrane domains calculated by TMHMM 2.0 software. NI, no transmembrane domain identified.

√ indicates that the number of unique peptides detected with each of the TAP-tagged constructs is higher than 2. ND, protein has not been detected.

Proteins identified in L. tarentolae and C. fasciculata cyt c oxidase complexes (11, 33).

Used as bait with TAPMIX.

Used as bait with TAPCOXIV.

Used as bait with TAPCOXV.

Used as bait with TAPTb2320.

ⁱ

Used as bait with TAPTb1900.

Trypanosomatid-specific protein.