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. 2008 Aug 22;7(11):1906–1915. doi: 10.1128/EC.00148-08

FIG. 1.

FIG. 1.

Targeting of GFP reporters by the C terminus of Hwp1p or Ecm331p. (A) C. albicans CAI4 cells expressing the Hwp1/GFP/Hwp1c, Hwp1/GFP/Ecm331c, Ecm331/GFP/Hwp1c, or Ecm331/GFP/Ecm331c fusion protein were fluorescent at or near the cell surface. (B) Western blots of laminarinase-digested CW probed with antibodies to GFP showed that the Hwp1/GFP/Hwp1c and Ecm331/GFP/Hwp1c fusions targeted mostly to the CW, whereas the Hwp1/GFP/Ecm331c and the Ecm331/GFP/Ecm331c fusions did not. (C) Triton X-114 extraction of the whole-cell lysates into aqueous (lanes A) and detergent (lanes D) fractions showed that both fusion proteins were present in the membrane-rich detergent phases. The arrows in B and C indicate the expected monomeric and dimeric forms of the proteins of interest, and molecular sizes in kDa are shown at the left. Hwp1c and Ecm331c represent Hwp1/FlagGFP/Hwp1c and Hwp1/FlagGFP/Ecm331c, respectively.