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Immunoblot analysis confirms that RαAasP recognizes recombinant AasP (rAasP) (A) and the corresponding ca. 104-kDa AasP protein in whole-cell lysates (B) and secreted proteins (C) of wild-type (WT) A. pleuropneumoniae 4074 but not in the ΔaasP mutant derivative. No additional AasP-specific bands are apparent, suggesting that intact AasP is the active form of the protein.
