FIG. 2.

Identifying cell types in the lung by flow cytometry. Cell types were identified based on differential expression of F4/80, CD11b, CD11c, and GR-1. Mouse lungs were digested with dispase, and cells were stained with fluorescently labeled cell-type-specific antibodies. (A, B) F4/80⁺ cells (A) that were CD11b^low (B) were classified as alveolar macrophages, while F4/80^high CD11b^high cells were classified as CD11b^high macrophages. (C) F4/80^low CD11c^high cells were classified as DCs and subdivided into CD11b^low/mid DCs and CD11b^high DCs. F4/80^low CD11c^low CD11b^mid cells were classified as monocytes. (D) F4/80^low CD11c^low CD11b^high GR-1^high cells were classified as neutrophils.