TABLE 2.

Primers and PCR conditions used in this study

Primer	Nucleotide sequence (5′→3′)a	PCR conditions (30 cycles)			Amplicon size (bp)
		Denaturing	Annealing	Extension
stx2Aseq_F1	TACCAGGCTCGCTTTTGCGG	94°C, 30 s	60°C, 45 s	72°C, 80 s	1,110
stx2Aseq_R2	CGCCATTGCATTAACAGAAGC
stx2Aseq_F2	CCATGACAACGGACAGCAG
stx2Aseq_R1	CTGTATCTGCCTGAAGCGTAAGGC
stx2Bseq_F	CCAGAATGTCAGATAACTGGC	94°C, 30 s	58°C, 45 s	72°C, 30 s	476
stx2Bseq_R	GGCAACTGTCAACTGACTG
curli1F	CGCTTAAACAGTAAATGCCG	98°C, 10 s	60°C, 30 s	72°C, 35 s	1,046
curli1R	CCGCATGGTGACCAACGA
curli2F	TTCCTTATGAAGCTGGGGC	98°C, 10 s	60°C, 30 s	72°C, 35 s	1,054
curli2R	CGGAATCAGCCCTCCTTAC
curli3F	CGCTGATGAACAACGAACG	98°C, 10 s	60°C, 30 s	72°C, 35 s	1,028
curli3R	CCCGTCGCTGATTGCTGC
curli4F	CTCCACACCACCGTGGAC	98°C, 10 s	60°C, 30 s	72°C, 35 s	1,047
curli4R	GCTTGCAGAGCAAGTGCAG
curli5F	GGCAGGTGTTGTTCCTCAG	98°C, 10 s	60°C, 30 s	72°C, 35 s	886
curli5R	CCTTGAGGGTTGTGTTATCC
csgBACprom_F	cgcggatccGTTGTACATTTGGTTTTTATTGCAC	94°C, 15 s	60°C, 30 s	72°C, 60 s	524
csgBACprom_R	cggggtaccCAATTTGTTTTTCATGTTGTCACC
F1upCsgBA	aagagctcGCACACCTGACAGCTGCC	94°C, 30 s	60°C, 30 s	72°C, 60 s	739
F1downCsgBA	aaggatccTCACCCTGGACCTGGTCG
F2upCsgBA	aaggatccCGCGACCGCTCATCAGTAC	94°C, 30 s	60°C, 30 s	72°C, 60 s	960
F2downCsgBA	aactgcagTTCATAACGCCTCCTTACACC
CsgBAC1	aaggatccGTTGCGTTAACAACCAAGTTG	94°C, 30 s	60°C, 30 s	72°C, 90 s	1,016
CsgBAC2	aaggtaccGCTGATTCGCAGCAGACCA

^aThe lowercase portions of some sequences are nonmatching, and the underlined bases show the incorporation of restriction sites.