TABLE 2.
Primer | Nucleotide sequence (5′→3′)a | PCR conditions (30 cycles)
|
Amplicon size (bp) | ||
---|---|---|---|---|---|
Denaturing | Annealing | Extension | |||
stx2Aseq_F1 | TACCAGGCTCGCTTTTGCGG | 94°C, 30 s | 60°C, 45 s | 72°C, 80 s | 1,110 |
stx2Aseq_R2 | CGCCATTGCATTAACAGAAGC | ||||
stx2Aseq_F2 | CCATGACAACGGACAGCAG | ||||
stx2Aseq_R1 | CTGTATCTGCCTGAAGCGTAAGGC | ||||
stx2Bseq_F | CCAGAATGTCAGATAACTGGC | 94°C, 30 s | 58°C, 45 s | 72°C, 30 s | 476 |
stx2Bseq_R | GGCAACTGTCAACTGACTG | ||||
curli1F | CGCTTAAACAGTAAATGCCG | 98°C, 10 s | 60°C, 30 s | 72°C, 35 s | 1,046 |
curli1R | CCGCATGGTGACCAACGA | ||||
curli2F | TTCCTTATGAAGCTGGGGC | 98°C, 10 s | 60°C, 30 s | 72°C, 35 s | 1,054 |
curli2R | CGGAATCAGCCCTCCTTAC | ||||
curli3F | CGCTGATGAACAACGAACG | 98°C, 10 s | 60°C, 30 s | 72°C, 35 s | 1,028 |
curli3R | CCCGTCGCTGATTGCTGC | ||||
curli4F | CTCCACACCACCGTGGAC | 98°C, 10 s | 60°C, 30 s | 72°C, 35 s | 1,047 |
curli4R | GCTTGCAGAGCAAGTGCAG | ||||
curli5F | GGCAGGTGTTGTTCCTCAG | 98°C, 10 s | 60°C, 30 s | 72°C, 35 s | 886 |
curli5R | CCTTGAGGGTTGTGTTATCC | ||||
csgBACprom_F | cgcggatccGTTGTACATTTGGTTTTTATTGCAC | 94°C, 15 s | 60°C, 30 s | 72°C, 60 s | 524 |
csgBACprom_R | cggggtaccCAATTTGTTTTTCATGTTGTCACC | ||||
F1upCsgBA | aagagctcGCACACCTGACAGCTGCC | 94°C, 30 s | 60°C, 30 s | 72°C, 60 s | 739 |
F1downCsgBA | aaggatccTCACCCTGGACCTGGTCG | ||||
F2upCsgBA | aaggatccCGCGACCGCTCATCAGTAC | 94°C, 30 s | 60°C, 30 s | 72°C, 60 s | 960 |
F2downCsgBA | aactgcagTTCATAACGCCTCCTTACACC | ||||
CsgBAC1 | aaggatccGTTGCGTTAACAACCAAGTTG | 94°C, 30 s | 60°C, 30 s | 72°C, 90 s | 1,016 |
CsgBAC2 | aaggtaccGCTGATTCGCAGCAGACCA |
The lowercase portions of some sequences are nonmatching, and the underlined bases show the incorporation of restriction sites.