FIG. 2.
Purification of BTDs from leukocytes. (A) Acid extracts of peripheral blood leukocytes were chromatographed using a Bio-Gel P-60 gel filtration column (inset) equilibrated in 5% acetic acid. Fractions denoted by the bracket contain θ-defensin molecular masses. These fractions were pooled and rechromatographed by semipreparative RP-HPLC on a Vydac C18 column (10 by 250 mm). Numbers above the peaks indicate baboon θ-defensins. (B) BTD-1, -2, -3, -4, and -7 were purified to homogeneity, and 0.5 μg of each BTD was observed by analytical C18 RP-HPLC. BTD-2 is synthetic.