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Western blotting of baboon and rhesus θ-defensins. Acid extracts of 4 × 106 baboon leukocytes (lane 1), 1 μg each of synthetic BTD-1, -2, -3, -4, and -7 (lanes 2 to 6, respectively), and a mixture of RTD-1, -2, and -3 (lane 7) were resolved on a 12.5% AU-polyacrylamide gel, transferred to a nitrocellulose membrane, and immunoblotted with goat anti-RTD-1 IgG.
