FIG. 8.
E. coli ML35 permeabilization by BTDs. (A) Synthetic BTDs were incubated with 2 × 106 CFU E. coli in 10 mM PIPES (pH 7.4) and 3 mM ONPG, and ONP efflux was monitored spectrophotometrically at 405 nm. (B) BTD inhibition of purified β-galactosidase (β-gal) was determined by monitoring the rates of ONPG hydrolysis in a cell-free system. (C) The adjusted rates of ONPG hydrolysis were calculated using the inhibition coefficients determined from panel B to correct the values in panel A. Results shown are representative of duplicate experiments.