TABLE 2.
Isolation of RASV strains from chicken tissues 3 days following the first inoculation
| Vaccine strain | No. of organisms isolated from tissues 3 days after immunizationa
|
||
|---|---|---|---|
| Spleen | Bursa | Cecum | |
| None (unimmunized) | 0 | 0 | 0 |
| χ8879(pYA3870) (T3SS-vector control) | 1.9 ± 1.60 (1)b | 5.6 ± 0.93 | 7.2 ± 0.56 |
| χ8879(pYA4157) (T3SS-SO7) | 0.7 ± 1.21 (2) | 5.4 ± 1.06 | 6.0 ± 0.50 |
| χ9242(pYA4184) (T2SS-SO7) | 0.9 ± 1.61 (2) | 5.2 ± 0.38 | 6.8 ± 0.43 |
a
Log10 geometric mean colony count per gram of tissue ± standard error obtained for three chickens. Groups of 21 chicks were inoculated when they were 7 days old with S. enterica serovar Typhimurium χ8879 (ΔsptP1033::xylE ΔasdA16 ΔphoP233) harboring plasmid pYA3870 or pYA4157 or with S. enterica serovar Typhimurium χ9242 (ΔphoP233 ΔasdA16 ΔaraBAD23 ΔrelA198::araC PBAD lacI TT) harboring pYA4184.
b
The numbers in parentheses are the numbers of chicks with less than 10 CFU/g of tissue.