TABLE 1.
Strain or plasmid | Description | Source or reference |
---|---|---|
Strains | ||
MMH594 | Clinical blood isolate | 16 |
E99 | Clinical urine isolate | 39 |
DBS01 | perA insertion mutant | This study |
DBS01(pGT101) | Contains plasmid pGT101 (perA insertion mutant complemented with plasmid-encoded perA) | This study |
Plasmids | ||
p3ERM | Suicide vector (erythromycin resistance marker) | 2, 14 |
p3ERMperA | Suicide vector used to generate a SCO mutant deficient in perA expression (DBS01) | This study |
pAT28 | Shuttle vector (spectinomycin resistance marker) | 40 |
pGT101 | pAT28 containing a full-length copy of the perA gene to complement the mutant strain DBS01 | This study |