TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Description	Source or reference
Strains
MMH594	Clinical blood isolate	16
E99	Clinical urine isolate	39
DBS01	perA insertion mutant	This study
DBS01(pGT101)	Contains plasmid pGT101 (perA insertion mutant complemented with plasmid-encoded perA)	This study
Plasmids
p3ERM	Suicide vector (erythromycin resistance marker)	2, 14
p3ERMperA	Suicide vector used to generate a SCO mutant deficient in perA expression (DBS01)	This study
pAT28	Shuttle vector (spectinomycin resistance marker)	40
pGT101	pAT28 containing a full-length copy of the perA gene to complement the mutant strain DBS01	This study