TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′-3′) | Description |
|---|---|---|
| BBA21F | GATAAATAAAAGAAATTTATCTGAAA | Primer pair that amplifies a 1.7-kb fragment containing bba21 to sequences downstream of bba24 (dbpA) |
| dbpAsalmut | ACGCGTCGACATCCTTCTTTTACTGATGA | |
| BBA31com | CGCATTTGCAAGAGAATCAAGCGCATCGTCTT | Primer pair that amplifies a 2.8-kb fragment containing bba31 to sequences upstream of bba25 (dbpB) |
| dbpBsalmut | ACGCGTCGACCAAGACCATAACTATTGAATT | |
| 5pflgGentSal | ACGCGTCGACGAACGAATTGTTAGGTGGCGGTACT | Primer pair that amplifies a 0.9-kb fragment containing the aacC1 allele (Gentr) fused to the borrelial flgB promoter (PflgB-Gentr) in the ΔdbpBA::Gentr mutant only |
| 3pflgGentSal | ACGCGTCGACCCCGAGCTTCAAGGAAGATTTCCT | |
| 5ABMut | TTTATGTCTTGATTATCGGGCGAAGAGTTTA | When used with 3ABMut, amplifies 1.6- and 1.4-kb bands in the parent and ΔdbpBA::Gentr mutant, respectively; when used with 5pflgGentSal, amplifies a 1.1-kb fragment in the ΔdbpBA::Gentr mutant only |
| 3ABMut | AAGCCAGATTGCATAGCAAGCTTGAATTCCAA | When used with 3pflgGentSal, amplifies a 1.2-kb fragment in the ΔdbpBA::Gentr mutant only |
| ATTR1F | GCATGCCCTCGAATCAACAAGTTTG | Primer pair used to amplify the attR1-Camr-ccdB-attR2 region from pDEST17 for cloning into borrelial shuttle vectors to convert them to Gateway amenable vectors |
| ATTR1R | GCATGCCGCAGCCTCGAATCAACCAC | |
| DbpBA-F-XmaNco | CCCGGGCCATGGCTTGATTATCGGGCGAAGAG | Primer pair that amplifies a 1.7-kb fragment containing the intact dbpBA locus with 289 bp upstream and 131 bp downstream for cloning into pCR8/GW/TOPO |
| DbpBA-R-XmaNde | CCCGGGCATATGGCAAACTGGAAACAAGTC | |
| nTM17FrecA | GTGGATCTATTGTATTAGATGAGGCTCTCG | Primer pair used for real-time quantitative PCR detection of B. burgdorferi recA genea |
| nTM17RrecA | GCCAAAGTTCTGCAACATTAACACCTAAAG | |
| bactinF | CAAGTCATCACTATTGGCAACGA | Primer pair used for real-time quantitative PCR detection of the murine β-actin geneb |
| bactinR | CCAAGAAGGAAGGCTGGAAAA |