TABLE 3.
ID50 of the ΔdbpBA::Gentr strain, JF105/pBBE22, in immunocompetent C3H mice
| Isolate | Inoculum (cells) | No. of cultures positive/total no.
|
No. of mice positive/total no. | Calculated ID50 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Lymph node | Skind | Heart | Spleen | Bladder | Joint | All sites | ||||
| ML23/pBBE22a | 104 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 12/12 | 2/2 | ∼150e |
| 103 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | 24/24 | 4/4 | ||
| 102 | 1/3 | 1/3 | 1/3 | 1/3 | 1/3 | 1/3 | 6/18 | 1/3 | ||
| JF105/pBBE22b | 106 | 3/3 | 0/3 | 0/3 | 0/3 | 1/3 | 1/3 | 5/18 | 3/3 | >106 |
| 105 | 2/5 | 1/5 | 0/5 | 0/5 | 0/5 | 0/5 | 3/30 | 2/5 | ||
| 104 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/18 | 0/3 | ||
| 103 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/6 | 0/36 | 0/6 | ||
| 102 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/18 | 0/3 | ||
| JF105/pJBF17c | 105 | 2/3 | 3/3 | 3/3 | 2/3 | 3/3 | 3/3 | 16/18 | 3/3 | 316 |
| 104 | 3/3 | 2/3 | 3/3 | 0/3 | 3/3 | 3/3 | 14/18 | 3/3 | ||
| 103 | 4/5 | 4/5 | 4/5 | 0/5 | 4/5 | 4/5 | 20/30 | 4/5 | ||
| 102 | 2/3 | 0/3 | 1/3 | 1/3 | 1/3 | 1/3 | 6/18 | 2/3 | ||
a
Parental strain B31 derivative which provides BBE22 in trans and is infectious (46).
b
ΔdbpBA::Gentr derivative of ML23/pBBE22.
c
ΔdbpBA::Gentr derivative of ML23/pBBE22 complemented with intact and expressed dbpBA.
d
The skin tested was skin from the site of inoculation.
e
The ID50 of ML23/pBBE22 is based on the results of numerous reproducible infectivity studies (31).