FIG. 3.

Overexpression of iNOS in PDL cells increased AFn-mediated nitric oxide production. (A) PDL cells were transfected with cDNA for iNOS at two different doses or with a vector control and then treated with AFn for 5 h, and the nitric oxide generated into the medium was measured. M, cells treated with serum-free medium only; *, P value of <0.05 compared with results for vector-transfected and medium-treated cells. (B) The cells used in the experiment described for panel A were lysed, and the lysate used to analyze iNOS by Western blotting. Actin was used as a loading control. M, cells treated only with serum-free medium; V, vector-transfected cells.