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. 2008 Oct 6;76(12):5514–5523. doi: 10.1128/IAI.00625-08

FIG. 4.

FIG. 4.

Inhibition of JNK-1 inhibited nitric oxide generation in PDL cells treated with AFn fragment. (A) PDL cells were transfected with two different doses of siRNA for JNK-1 or a control siRNA (CT-siRNA) and then treated with AFn or serum-free medium (M), and the nitric oxide in the medium was assayed by using Griess reagent. *, P value of <0.05 compared with results for control siRNA-transfected and medium-treated cells; #, P value of <0.05 compared with results for control siRNA-transfected and AFn-treated cells. (B) The cells used for the experiment described for panel A were lysed, and the levels of iNOS, pJNK-1, and JNK-1 were determined by Western blot analysis. Actin was used as a loading control. CT, control untransfected cells; CT si, control siRNA-transfected cells; M, serum-free medium.