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. 2008 Sep 29;76(12):5777–5789. doi: 10.1128/IAI.00676-08

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FIG. 4. — Localization of the LptA protein in N. meningitidis. Whole-cell lysates (WC) and the cytoplasmic and periplasmic fraction (C+P), the inner-membrane-enriched fraction (IM), and the outer-membrane-enriched fraction (OM) prepared by sonication from N. meningitidis strain H44/76 (A and C) or HT1295 (B and C) were separated and analyzed by Western blotting. (A and B) Five microliters of each sample was analyzed by Western blotting with anti-LptA rabbit antiserum (A) or with anti-FLAG monoclonal antibody (mAb) (B). (C) One microliter of each sample was analyzed to detect the Opc protein by Western blotting with monoclonal antibody B306 (1) as an outer membrane protein marker.