TABLE 2.
Oligonucleotides used in this study
| Primer | Target gene | Nucleotide sequence (5′→3′)a |
|---|---|---|
| NMB1638-pET24a-5 | lptA | CGggatccTACGCCTCGTTTTTCCGCAACAATAAATCA |
| NMB1638-pET24a-2 | lptA | CCGctcgagTGCGCGGACGGCGGCAGGCTGCCAA |
| lptA-1 | lptA | ACGCAGGGTTCAAGATGAGTTGAA |
| lptA-2 | lptA | CCCAGCAAACCTGCCTATCCGAAA |
| lptA-13 | lptA | GCCGGATATGCCGTCTGAAGCCTTCGG |
| lptA-14 | lptA | AActgcagCCGGATACCGCTACCACAACTGGGG |
| lptA-15 | lptA | GCCGCTGCTTGCCGCGCGCGGCGA |
| lptA-16-FLAG | lptA | GGGgttaacTTACTTGTCGTCATCGTCTTTGTAGTCGCGCGGGCAGGCTGCCAATATATC |
a
Lowercase indicates additional nucleotides used to create the restriction sites. The underlined nucleotides were originally added to attach the FLAG epitope (see Materials and Methods).