FIG. 4.

Ex vivo levels of cytokines in ankle tissue from control C3H (wild type) and C3H IL-10^−/− (IL-10 ko) mice following infection with B. burgdorferi. The mice were sacrificed on day 21 postinfection, and one knee joint was removed immediately upon sacrifice and frozen in liquid nitrogen as described in Materials and Methods. Samples were homogenized, and the levels of IL-1β, IL-12p70, IL-4, and KC were determined by ELISA. The data are means plus standard errors expressed as pg cytokine/mg total protein and are pooled data from two separate experiments; n = 10. An asterisk indicates that the IL-10 ko value is significantly different from the wt value at the P < 0.002 level.