TABLE 1.
Bacterial strains and plasmids
| E. coli strains and plasmids (source) | Descriptiona | Origin or reference |
|---|---|---|
| E. coli strains | ||
| Genehogs | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80ΔlacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Smr) endA1 nupG fhuA::IS2 (confers phage T1 resistance) | Invitrogen |
| C600 | K-12, stx negative, q negative, O rough; Lac− Trp+ His+ | 48 |
| CB2851 | Wild-type O157:H7, stx2c | 48 |
| TPE1874 | C600, lysogenic for phage 2851 | 48 |
| TPE1875 | C600, lysogenic for phage 2851 | 48 |
| Plasmids | ||
| pLitmus38 | 2.2-kb cloning vector; Apr | New England Biolabs |
| pMS470Δ8 | 6.9-kb cloning vector, pMB1 replicon, Ptac, lacI; T7 gene Shine-Dalgarno sequence; Apr | 4 |
| pJH701 (CB2851) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage cI, PCR NdeI-HindIII] | This work |
| pJH701X (CB2851) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 2851 cI negative, PCR NdeI-HindIII] | This work |
| pJH702 (CB2851) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 2851 cII, PCR NdeI-HindIII] | This work |
| pJH702X (CB2851) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 2851 cII negative, PCR NdeI-HindIII] | This work |
| pJH703 (CB2851) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 2851 antA, PCR NdeI-HindIII] | This work |
| pJH703X (CB2851) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 2851 antA negative, PCR NdeI-HindIII] | This work |
| pJH704 (CB2851) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 2851 antB, PCR NdeI-HindIII] | This work |
| pJH704X (CB2851) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 2851 antB negative, PCR NdeI-HindIII] | This work |
| pJH705 (TPE1874)b | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 1874 antAB, PCR NdeI-HindIII] | This work |
| pJH705X (TPE1874) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 1874 antAB negative, PCR NdeI-HindIII] | This work |
| pJH707 (CB2851) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 2851 cro, PCR NdeI-HindIII] | This work |
| pJH707X (CB2851) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 2851 cro negative, PCR NdeI-HindIII] | This work |
| pJH709 (CB2851) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 2851 ospB, PCR NdeI-HindIII] | This work |
| pJH709X (CB2851) | pMS470Δ8 Δ[NdeI HindIII] Ω[phage 2851 ospB negative, PCR NdeI-HindIII] | This work |
a
Apr, ampicillin resistance; Smr, streptomycin resistance.
b
antAB fusion gene generated from 5′ region of antA and 3′ region of antB (see text).