TABLE 2.
Nonhuman primate immunoglobulin directed against plague vaccine antigens
| Group | Antigena | Log10 titers for antigen-specific immunoglobulin isotypes (mean ± SD)
|
|
|---|---|---|---|
| IgG1 | IgM | ||
| 1 | PBS | NDb | ND |
| 2 | rLcrV | 3.04 ± 2.71 | 3.29 ± 2.99 |
| 3 | rV10 | 2.98 ± 2.73 | 3.32 ± 2.86 |
| 4 | rF1 | 2.97 ± 2.60 | 3.41 ± 3.27 |
| 5 | rLcrV + (rF1) | 2.65 ± 2.38 | 3.27 ± 2.06 |
| 5 | (rLcrV) + rF1 | 2.79 ± 2.61 | 3.20 ± 2.23 |
| 6 | rV10 + (rF1) | 2.88 ± 2.30 | 3.24 ± 2.37 |
| 6 | (rV10) + rF1 | 2.79 ± 2.10 | 3.17 ± 2.78 |
a
Cynomolgus macaques (see Table 3 for animal group assignments) were immunized by intramuscular injection of 50 μg antigen adsorbed to aluminum hydroxide adjuvant (Alhydrogel) on days 0, 21, and 42. The monkeys were bled at day 49 postimmunization. Specific IgG1 and IgM were detected by ELISA in serum for individual monkeys.
b
ND, rLcrV or rF1 antigen-specific immunoglobulin was not detected.