FIG. 1.
Fusion protein expressed on the surface of recombinant S. gordonii GP1456. (A) Schematic structure shows the fusion protein displayed on the surface of S. gordonii GP1456. The recombinant protein consists of OVA323-339 (ISQAVHAAHAEINEAGR, black area), OVA257-264 (SIINFEKL, black area), and the Ag85B-ESAT-6 fusion protein (388 amino acids, gray area). (B) Flow cytometry analysis of S. gordonii GP1456 (gray histogram) and control strain (open histogram). Bacterial cells were reacted with Ag85B-ESAT-6-specific mouse serum and then with fluorescein isothiocyanate-conjugated anti-mouse immunoglobulin G.