FIG. 5.

Time course analysis of the clonal expansion of OT-I CD8⁺ and OT-II CD4⁺ T cells following intranasal immunization with recombinant S. gordonii. The clonal expansion of adoptively transferred OT-I CD8⁺ and OT-II CD4⁺ T cells following intranasal immunization with recombinant vaccine strain GP1456 or control strain GP1295 was analyzed on days 3, 5, and 7 postimmunization in cervical lymph nodes (CLN), lung, and spleen. Transgenic OT-I and OT-II T cells were identified on the gated CD8⁺ CFSE-positive and CD4⁺ CFSE-positive populations, respectively, with light scatter properties of lymphocytes. The percentages of proliferating cells are shown on the y axis. Bars represent the means ± standard errors of the means of values from nine mice assessed in three separate experiments.