FIG. 7.

Ag85B-ESAT-6-specific T-cell proliferative response and IFN-γ production. Mice were immunized by the nasal route with a single dose of GP1456 or control strain GP1295, and the Ag85B-ESAT-6-specific T-cell response was assessed in splenocytes 5 days after the inoculum. (A) Ag85B-ESAT-6-specific proliferation of splenocytes was assessed by tritiated thymidine incorporation. (B) IFN-γ production of splenocytes was restimulated with 20 μg/ml of Ag85B-ESAT-6. Bars represent the means ± standard errors of the means of values from 10 mice assessed in two separate experiments. **, P ≤ 0.01; ***, P ≤ 0.0001 versus control.