TABLE 1.
Binding of defensins to adhesins of Porphyromonas gingivalisa
| Ligand and analyte | Constantd
|
Relative response (RU) at concn:
|
|||||
|---|---|---|---|---|---|---|---|
| Ka | Kd | KD | 1 μg | 10 μg | 50 μg | 100 μg | |
| rHagBb | |||||||
| HBD1 | 2.14E+03 (1.14E+03) | 1.37E−05 (2.67E−06) | 8.76E−09 (2.30E−09) | 0.0 (0.0) | 0.0 (0.0) | 13.3 (13.3) | 0.0 (0.0) |
| HBD2 | 1.76E+05 (1.14E+05) | 9.74E−03 (4.86E−03) | 5.00E−08 (2.95E−08) | 9.3 (4.0) | 21.8 (6.1) | 49.5 (20.6) | 333.2 (227.9) |
| HBD3 | 1.83E+04 (1.73E+04) | 6.00E−04 (5.90E−04) | 1.79E−08 (7.83E−09) | 28.7 (18.4) | 345.7 (107.8) | 2,197.0 (635.8) | 2,586.5 (1,278.0) |
| HNP-1 | 1.64E+06 (5.63E+05) | 1.28E−02 (1.11E−03) | 1.08E−08 (4.41E−09) | 77.5 (41.7) | 230.8 (64.2) | 478.2 (65.0) | 675.1 (22.3) |
| HNP-2 | 1.26E+07 (6.15E+06) | 1.38E−02 (8.88E−04) | 1.66E−09 (5.94E−10) | 74.3 (37.1) | 242.6 (42.9) | 531.8 (30.4) | 809.2 (35.9) |
| rFimAc | |||||||
| HBD1 | 4.16E+06 (2.23E+06) | 1.96E−03 (1.17E−03) | 3.70E−09 (3.25E−09) | 192.0 (192.0) | 218.4 (218.4) | 201.0 (154.6) | 237.5 (132.9) |
| HBD2 | 1.16E+10 (1.14E+10) | 6.53E−02 (3.20E−02) | 4.08E−10 (3.01E−10) | 194.3 (188.6) | 260.6 (205.1) | 453.0 (139.3) | 635.8 (103.3) |
| HBD3 | 2.78E+04 (8.24E+03) | 1.14E−02 (4.51E−04) | 4.88E−07 (1.32E−07) | 424.2 (153.5) | 2,324.0 (314.9) | 5,361.9 (342.8) | 7,316.3 (130.7) |
| HNP-1 | 1.57E+04 (2.72E+03) | 6.83E−03 (2.02E−04) | 4.60E−07 (7.47E−08) | 232.2 (184.2) | 4,878.8 (486.9) | 12,012.0 (537.1) | 16,259.0 (323.0) |
| HNP-2 | 1.32E+05 (8.52E+04) | 8.17E−03 (4.15E−04) | 2.27E−07 (1.69E−07) | 332.7 (182.7) | 5,269.9 (791.5) | 13,948.3 (574.5) | 19,730.8 (352.3) |
a
Values are shown as means (standard errors of the means).
b
Recombinant hemagglutinin B (rHagB) adhesin of P. gingivalis strain 381.
c
Recombinant fimbrillin A (rFimA) adhesin of P. gingivalis strain 381.